This arrangement was inconvenient; furthermore, inactivation of the enzymes was not complete even at this low temperature, and some denaturation of the proteins took place as evidenced by the gradual appearance of insoluble precipitates in the stored vials. [Please select]
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Formalin caused slight denaturation of some of the proteins, but this effect was complete within a few hours, after which any denatured material was removed by filtration or centrifugation. [Please select]
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Formolization of the antigens was necessary as a means of preventing denaturation of the proteins by enzymatic activity. [Please select]
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